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The principles of separation in both techniques are similar with two 'phases' being employed.The instrument consists of a column to which a stationary phase is bonded.
In DESI, the same solvents as used for conventional ESI are electrosprayed onto the surface containing the analyte of interest.
An advantage of DESI is that the removal of the sample preparation step makes the technique suitable for high-throughput analyses, such as checking luggage for traces of drugs and explosives at airports.
The advances in MS have not just been restricted to ionisation techniques.
He demonstrated that large molecules in excess of 130,000 Da could be ionised using his new technique.
The combination of MALDI and ESI paved the way for structural biologists to analyse large proteins to understand how biological systems work.